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His Tag anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, IP, IHC (fro) et EIA. Il présente une réactivité envers .
N° du produit ABIN119353

Aperçu rapide pour His Tag anticorps (ABIN119353)

Antigène

Voir toutes His Tag Anticorps
His Tag

Reactivité

Veuillez nous consulter SVP

Hôte

  • 110
  • 66
  • 11
  • 10
  • 9
  • 8
  • 2
  • 1
Souris

Clonalité

  • 125
  • 77
  • 11
Monoclonal

Conjugué

  • 96
  • 22
  • 14
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  • 5
  • 4
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  • 3
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
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Cet anticorp His Tag est non-conjugé

Application

  • 171
  • 120
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  • 16
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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone

AD1-1-10
  • Séquence

    HHHHHH

    Purification

    Affinity Chromatography on Protein G

    Immunogène

    PAX6 transcription factor linked to histidine tag. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

    Isotype

    IgG1
  • Indications d'application

    Immunhistology on Frozen Sections. ELISA. Immunoprecipitation. Western Blot (1/500-1/1000). In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS containing 0.09 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    His Tag

    Autre désignation

    6xHistidine Epitope Tag

    Classe de substances

    Tag

    Sujet

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.
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